FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_62.351000000395ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_62.351000000395ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084571
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGGG402.468976E-422.4978569
GCTGACC600.003756119614.998578
CTGTGCG600.003756119614.998579
CGTCGTG1054.265279E-614.28698730
CGTGAGC952.6383806E-514.20851712
TAGGGGG1106.8955906E-613.6388384
GTATTAG2900.013.4513361
TAGGTAG1551.1294469E-712.5829285
TAGGGAG1101.0410283E-412.2749545
ACCTAAT2351.2732926E-1112.1304191
CCTAATA2600.012.1175822
CTAATAC2750.012.0016243
GCGTGCT1006.456117E-411.99830311
TGGAGTG1652.5475674E-711.8203255
TCCTAGG1151.5755779E-411.7412612
ACGTGAG1151.5803192E-411.73747211
CATATAC4200.011.4301183
CCGTCGT1059.7027724E-411.42958929
TATACAC3450.011.3058783
ACACAGC1202.3399349E-411.252046
Produced by FastQC (version 0.11.2)