FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_61.351000000395ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_61.351000000395ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514982
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC952.6289617E-514.2146853
ACTATGC4450.013.8192528
ATCACAC2900.013.44821629
TAGGGGG800.001653183513.1288424
TGGGGGG1855.0567905E-1012.976775
ATACCGA2700.012.22586
TCCTAGG1006.431631E-412.0043062
ACCGTGT1006.454874E-411.9991558
CTATGCA3950.011.7713239
ACCTAAT4350.011.3848981
TACTATG5400.011.1095957
CTCCTAA1501.3526405E-511.0054021
TCACACA3300.010.90904230
AGGGGGC1100.001433981210.9083238
GTATATA12650.010.7958131
GACTCCT1408.1884486E-510.7128247
ACTGTCC2251.1013981E-810.66591558
CCTAATA5800.010.6072532
TAATACT7650.010.5913354
TAGGTAG1704.6620826E-610.5913345
Produced by FastQC (version 0.11.2)