FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_60.35100000039568.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_60.35100000039568.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685676
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTC350.003005269221.42815627
CTAGTAC805.160753E-616.8771343
TCCGAGC550.002100753416.36331713
CTCCGAG600.003752348714.99970812
CGAGACG600.003754169714.99861523
GTATTAG2100.014.2885631
TATTAGG1802.1827873E-1114.1684582
TAAGGTT1251.770919E-613.2016694
GGTAGTC800.001656372713.1237887
AGTACCT954.179131E-412.6331765
AGTCAGA954.184277E-412.63133410
TAGACAG850.002607933512.3545025
TCTACCC850.002607933512.3545023
GTGGCCA850.002610788712.3527019
GTATTAA2001.724402E-912.0023931
ATACGGT1754.2129614E-812.0015176
TACGGTG1406.309967E-611.7846267
AGTACAG1151.5747649E-411.7406155
GTCTAAG900.003998304311.6689931
CCACGAG900.004007030311.66558820
Produced by FastQC (version 0.11.2)