FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_59.35100000039525.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_59.35100000039525.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1172944
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCCGG500.001110506217.9993619
TACACAG1751.2732926E-1114.574645
CGGTCGG650.006387653313.84566213
TCTAGGA1106.897002E-613.6387862
GCCGCGT800.001656353713.12509325
AGGGTGC850.002608002612.3556626
GTCCTAA1501.0362764E-612.0026451
CGCCGCG1006.455691E-411.99855124
GTATATG4150.011.5688131
TACACTG1703.7505743E-711.4731155
CCCTTAT2259.076757E-1011.335831
CCGAGCC950.005998313411.05223914
TGGGGGG1653.262092E-610.9114945
GGTGCCG1100.001433144910.9087038
GTTCTAT2504.5110937E-1010.802381
TAGGTAG1704.6632013E-610.5905685
GTCTAGA1000.00876293510.5023141
TAGTCAG1000.0087795710.4996279
TATCCTG2453.7071004E-910.4104575
GCGTCAG1451.1638579E-410.3471071
Produced by FastQC (version 0.11.2)