FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_58.351000000394e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_58.351000000394e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744385
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGA653.4386845E-416.15131411
GTCTTAA1104.1823114E-715.00369551
CTGAACT1002.565761E-615.0026894
TAGAACT1206.844857E-815.0026884
GCCGCCT650.00638989313.84398311
CTAGAAC1004.2593645E-513.502423
CTTACAC1251.7704497E-613.2023653
CTGGAGA1056.732236E-512.8594474
TAGACTG954.1780173E-412.6338435
CTTATAC2550.012.3551543
TATACTG3050.012.2972855
TATACAG3200.011.7208495
GTCCATA900.00399744911.6695411
GACAGCT900.00401152211.6640557
CCTATAC1303.8322265E-511.5405293
GTGTTAG1703.7422433E-711.4734141
CCTTAAG1059.6837553E-411.4313881
CTGCTCT1059.716513E-411.426789
GTATAGG1859.1011316E-811.3541491
ACTATGC2301.3096724E-911.0852188
Produced by FastQC (version 0.11.2)