FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_57.351000000394ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_57.351000000394ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531015
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGC254.2257854E-429.9969868
ACTCCCG300.001225730424.99984420
CCGGCTC455.46494E-419.99799215
TTGACGG455.46494E-419.99799217
TAGGTAG904.245885E-818.3383985
GCCCCGG500.001109901817.99819212
CCCCGGC500.001109901817.99819213
AGGCCCC703.110007E-517.14113610
GTCTAGA550.00209444816.3697011
GGCCCCG550.0021010516.36199411
GTATTAC706.0123485E-415.0055591
GTCCTAA1002.5573117E-615.0055591
CCTTAAG901.5728467E-515.0055581
ACAGAGC600.003752886814.9984938
GTCCTAT1156.8944246E-714.3531441
GTCTAAT952.6207E-514.2157931
TAGGAGA650.00636841913.8499794
TCGCTTG650.006384333613.84476312
TTAGAGT1201.1155189E-613.7537994
TATTAGG1557.696144E-913.5521312
Produced by FastQC (version 0.11.2)