FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_56.35100000039469.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_56.35100000039469.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795089
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTAGG500.001107411218.0058141
TAGGTAG1206.845403E-815.0029565
GGTAGTC1251.1328302E-714.398317
TGGGGGG952.6282312E-514.2133275
TCTAGGG851.6297135E-414.1204313
CAGCGTG851.6332006E-414.1168789
GTATTAG2250.014.0045211
ACCTAGC650.006380752713.8471429
CCTTATA1853.45608E-1113.7865012
GTATAAG1507.337803E-813.0041981
ATAAGGG1404.4897206E-712.8596773
ATACGGT1404.4897206E-712.8596776
CTAATAC2255.456968E-1212.6691643
TACAGCG1201.6937442E-512.4985327
TAGGAGT1456.8702684E-712.416244
ATACAGC1951.1586963E-912.3101186
ACAGACC1101.0426483E-412.27205858
GTACTAC1354.1880176E-612.226171
GGTCTAA1006.4276415E-412.0038761
GTCTTAA1501.0336007E-612.0038751
Produced by FastQC (version 0.11.2)