FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_55.35100000039426.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_55.35100000039426.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083507
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG452.0426742E-523.3301057
TGGGGGG1604.9112714E-1115.0041545
CTCCAGG1156.9064845E-714.3544521
TTGGGGG1504.8748916E-914.0038794
TACACAG1955.456968E-1213.8499895
AGACCGC1201.1185948E-613.7538096
CTAATAC3850.013.64014153
ATACGAC1106.888771E-613.6401413
TAGGTAG1557.728886E-913.5521395
GTATTAG3250.013.3907971
GAGGGTA800.001658028713.1231829
AATACTA4750.012.6350785
TAGAACC2153.274181E-1112.5616174
CTAGAGC1201.6882314E-512.5034634
ACTATGC4350.012.4120748
TAGTCAG1354.2136526E-612.22053059
TAATACT6400.012.1908754
CCTGAAC1006.43345E-412.0033243
TCACACA2750.012.00110830
ATCACAC2701.8189894E-1211.66774429
Produced by FastQC (version 0.11.2)