FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_54.351000000393e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_54.351000000393e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392013
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG859.193094E-615.8847124
TGGGGGG1503.0377123E-1015.0022285
GCTCGAG750.001018928213.99855811
GTCCAGG650.006371555413.8511961
GCCTCAC954.1907746E-412.63073220
AGGGGGG1354.202584E-612.2240375
CCCCTAC900.00399692311.6709161
CGCCCGT900.004010895311.66546510
GTCCAGA1703.7455357E-711.4747661
GGTGCCC1059.721423E-411.4273948
ATACTGT5550.011.3530376
GACCGTC1602.2646582E-611.2488417
CCCTACA950.005991057511.0542742
GCCCGTC950.006002280411.05149411
ACTGTGC2104.571666E-810.7131828
TATACTG5500.010.6379445
CCGCCCG1000.00878534510.49891959
GTATAGG3450.010.4385821
ACCGTGT1150.00207710.4337088
CTGAGCT1304.9203873E-410.3835469
Produced by FastQC (version 0.11.2)