FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_53.351000000393ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_53.351000000393ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514121
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATACAGGCAACACGCAGATATAGGTGCGACG8800.171165931755365No Hit
GTATATATATATACATGAGAAGAACGGCATAGTGCG8590.16708129020211196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACT350.003006972621.4248211
GTGCGAC1450.019.65364329
GCCACTC400.00651229518.74671713
TCTAGGT500.00110532918.0091083
GGTGCGA1603.6379788E-1215.93625928
TTAGTAC859.139489E-615.8903913
TGCGACG1655.456968E-1215.45334230
TACACAG809.6935706E-515.007595
CCAGGCG600.003752103814.99883327
AGCTCGC706.036833E-414.99737411
CTAATAC1802.1827873E-1114.1738383
CGGAAGC851.6317524E-414.11654928
ACAACAC750.001012719914.0070853
CTAGTCT750.001017948113.99754924
CGGACTC650.00638300913.84507730
TAAGATT1004.241299E-513.5068314
AGTGCCT902.651408E-413.3309998
GCTCGCG800.001656422913.12270312
AGGTGCG1958.367351E-1113.07590627
CTTGTAG1056.703801E-512.8636493
Produced by FastQC (version 0.11.2)