FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_52.35100000039377.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_52.35100000039377.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975108
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGCG400.006513853618.7479829
TGGGGGG908.5428474E-716.6734225
CGTCCTA600.00375350614.99992330
TGCTCCG600.003756068214.99838357
CGAGACT851.6338262E-414.11685123
TTCTAGG1106.8816007E-613.6411912
GCCAGGA800.00165168613.1296462
TGCAGTC800.001657437513.1235877
TACACAG2255.456968E-1212.6718015
AGACTGG1006.4254954E-412.0048646
ACACAGG1252.5620397E-512.0048646
GTCCTAG1151.5719862E-411.7438891
TAGGGTG900.003994226511.6713955
ACTGTCC1059.717244E-411.42734058
TCACACT1202.3346198E-411.2545594
GGTGCGA1355.6454854E-511.11105428
TATAGTG3300.010.9135135
TTGGGGG1100.001427508110.9135124
CCTATAC1653.2550306E-610.9129523
ACGAGAC1100.001433019110.90847522
Produced by FastQC (version 0.11.2)