FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_51.35100000039334.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_51.35100000039334.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621729
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1750.015.4329995
GTGTCCG809.760441E-514.9992159
TAGGGGG1608.021743E-1014.0665354
TGGGGGG1608.021743E-1014.0665355
TCCGAGC1201.69518E-512.49934513
TAGGTAG2051.9281288E-1012.4425935
CCGAGCC1252.5765503E-511.99937214
GCCCACG1303.8478207E-511.53785718
ATAGGGG1703.7537393E-711.4731713
ACAGTGC2103.74348E-911.4279728
CCTATAC2900.011.3818723
GTATTAG5150.011.0711361
TTAGACT2402.6393536E-910.6280484
CTACACA3250.010.618434
TAATACC5150.010.4884444
TCTACAC3600.010.4190133
CTAGGAC1304.9064634E-410.3869533
TAGGCAG1451.16370284E-410.3477955
CTTAGAC2209.0023605E-810.2295753
CCTACAC1952.1954002E-610.0022523
Produced by FastQC (version 0.11.2)