FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_50.351000000392ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_50.351000000392ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940230
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGG400.006511967618.74883720
TAGGTAG1751.2732926E-1114.5736265
GGTAGTC1251.1335578E-714.3983417
GTATTAG3000.014.002111
TCTAGGA902.6477614E-413.3353422
TAATACT4200.012.859084
GGGTGCT1056.754511E-512.8556617
TATTAGG2255.456968E-1212.6685752
ACTATGC2750.012.5446768
TTAAGGC1101.04104176E-412.2745783
TAGTCAG1652.554243E-711.8174489
CCTTAAG1151.575601E-411.74091
TGGGGGG950.00598850711.0542985
GTCTTAG950.00598850711.0542981
CCTTGGG950.00598850711.0542983
TATACTG4100.010.9772645
CTACCCT1100.001430461110.9107364
AGGCTGA1100.001430461110.9107366
ATTAGGT2802.5465852E-1110.71593
TCTTATA3250.010.6169842
Produced by FastQC (version 0.11.2)