FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_49.351000000392af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_49.351000000392af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919126
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTGT1006.434232E-412.0040356
TAGACTG2103.7234713E-911.4324145
TAGGTAG1901.3193858E-711.0563485
GCGCGCC950.00600385611.05145221
TATTAGG4350.011.0381932
TGGGGGG3000.011.0036995
AGGGGGG2053.1899617E-810.97935
CTAAGAC2751.8189894E-1110.9127593
CCCTACA1253.4129646E-410.8036322
CCGCGAA1000.00878763410.49887813
GTCCTAC1200.002945902810.0038841
GTACCGT1952.1930682E-610.0033636
TAAACCG1653.881401E-510.0033635
TGTACCG1356.9381896E-410.0033635
GGCGAGG1653.895884E-59.99997426
CGTGTGG1653.90035E-59.99893121
CAGGGTG1200.00295865379.9986729
CAGATCG1653.9014674E-59.9986729
TACTATG4950.09.9986727
TCTATAC3800.09.8717383
Produced by FastQC (version 0.11.2)