FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_48.3510000003925f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_48.3510000003925f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287886
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACC400.00650174618.74858925
GTATTAG500.001104166418.0080281
CTCTTAT750.001011276214.0062461
GTACCAG650.0063519313.852331
TATGCTC650.006359249413.8499235
TCTACAC650.006359249413.8499233
ATAGACA650.00637390913.84511127
AGGCAAC650.00637390913.84511110
TCTTATA1201.1104748E-613.7537432
GTATAAA1106.8348327E-613.6424461
AATACAG800.001647779813.1285735
GTATTAT1151.0812109E-513.0492961
TATACAG1151.08315E-513.0470295
TTAATAC850.002598750612.3563033
ATACAGA850.002598750612.3563036
GTAATAA1101.0333512E-412.2782011
TAATACT1501.0266394E-612.0032664
ATACTGT1006.408441E-412.0032666
TATAGTA1006.408441E-412.0032662
AATACTA1151.5665607E-411.7423265
Produced by FastQC (version 0.11.2)