FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_47.3510000003921b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_47.3510000003921b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575517
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCA600.00375059815.00017452
GTATTAG1751.2732926E-1114.5728651
TGGACAG750.001016771914.0001635
GTCTAAG650.006376781513.8475171
TAGGGAG650.006380453713.8463135
GTCTAAA800.001653431313.1262931
GGACCAT800.001654513613.1251526
TACACAG1456.8746886E-712.4139375
GTACACA1006.4397237E-412.0001394
CTCTGTA1006.444429E-411.9990969
CTAATAC2553.6379788E-1211.7648423
ATATGGG900.004002582311.6668013
GTACTAG1059.6899195E-411.4296971
TCACCAG1059.6969755E-411.4287045
TACATAG1459.306517E-611.3794425
GAGTAGT1100.001429622610.9101661
CTATACT1807.842809E-710.8334594
GTCTTAT1253.4126246E-410.8010641
TTAAGGT1253.4153688E-410.8001254
TGACTGT1253.4153688E-410.8001256
Produced by FastQC (version 0.11.2)