FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_46.351000000391d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_46.351000000391d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392763
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG350.003000971921.4306625
CCCTCTA350.003003189821.4279359
CAGACTC400.006504160418.7494419
GTATTAG1102.3377652E-816.367321
GACCGAG653.430193E-416.15336626
ATAGGGT706.0198654E-415.0014643
TTAGGTA750.001015101714.0013674
GTTCAGA650.00636668913.8492691
TACACAG650.00637206313.8475065
CTGTAGC650.00637744113.8457429
CGAGGCT650.00637744113.84574229
CCACGAG650.00637744113.84574220
TATACGG1004.2526037E-513.5013185
GTATTAA1702.7959686E-812.355721
TCTATAG850.002605075212.3541463
TAATACA2001.715307E-912.0011714
ATTAGGT900.00399614311.6678063
GACAGTG900.00399994711.6663217
ACGGTGT900.00399994711.6663218
GTAATAC1303.8246733E-511.5395883
Produced by FastQC (version 0.11.2)