FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_45.35100000039193.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_45.35100000039193.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268011
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGG300.001221971525.0074651
TTATAGG559.522623E-519.096612
GTATTAG400.00648658118.75561
GTATTAA859.124793E-615.8870951
ACAAGGC851.6271108E-414.1165938
ATTAGGT650.00635662613.8502883
TTATACC650.00635662613.8502883
TATTAGG650.00635662613.8502882
ATACAAC800.001646935413.128926
CTCTATA954.1583425E-412.6353512
ATTATCC850.002597428212.356633
TATACAG1551.522978E-611.616375
ATAATAC1303.806276E-511.5419073
TATTATA1951.5015758E-811.5419072
TCTATAC1202.3249208E-411.253363
ATGGATT950.005963029311.0559324
TATACTG950.005963029311.0559325
TATACAC950.005963029311.0559323
TATACCA1253.3934976E-410.8032254
TATAATA2104.4767148E-810.7174852
Produced by FastQC (version 0.11.2)