FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_44.35100000039150.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_44.35100000039150.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425983
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG400.00649628718.7538171
AGTACTA805.1477273E-616.8784335
CAGTACT550.002095869516.3669664
ACTCCCG550.00210080916.36120420
GTGCTAA653.4249524E-416.1571331
TGGGGGG653.4249524E-416.1571335
CGGTTAA653.434157E-416.1514437
TAGTACT951.5003807E-615.7926884
CTTATAC1052.4615292E-715.71748353
GTATTAA706.0165056E-415.0030541
GACCGTC600.003752466514.997777
ACCGTCG706.032613E-414.9977698
ATTATAC1351.8368155E-814.4473843
AGACCGT1156.8981717E-714.3507466
TATACTG1054.245603E-614.2886215
TGAGTAT750.001017241613.99791910
AATGCAC750.001017241613.99791910
CCTAGAC650.00636875913.8489723
CTTATGC650.00636875913.8489723
TAGACCG1201.1149423E-613.7527995
Produced by FastQC (version 0.11.2)