FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_43.35100000039136.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_43.35100000039136.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329985
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTGT200.005140858230.0013666
TAATGCG455.4535497E-420.000914
GGGTATG400.006482134618.7593821
CGTGAGC400.006504871418.74801312
CGAAGAC400.006504871418.74801314
CTAACTC500.001107605818.0008183
CTTCTGT601.8534875E-417.5087571
GCCCACG550.002098860216.361919
TAACTCT859.159588E-615.8830774
TACTTAT650.00635224513.8530811
TAGAACA650.00637141713.8467844
AACGTGA650.00637781913.84468510
TCTTATA850.002595694712.3591221
CAATACT850.002604568112.3535044
ATTATAC1006.4250606E-412.00054553
CTATAAA1151.5645659E-411.7450051
CTATTAC900.00398182311.6725041
TATATAC1459.269494E-611.37982753
ATATTAC950.005959030211.0581621
TTATACT950.005979242311.0531343
Produced by FastQC (version 0.11.2)