FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_42.351000000390f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_42.351000000390f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896065
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGC350.003003210321.4315626
TGGGGGG1101.2259989E-917.7297465
AGCGCAC550.002102333116.36226710
GCGCACT653.4377488E-416.15249411
CTTACAC1553.092282E-1115.4860323
GACCGCG706.038853E-414.9987447
GACTGGT851.6339713E-414.1164667
AGGGGGG1004.2628555E-513.5018835
TAGGACT902.6477943E-413.3351944
TTGGGGG902.6477943E-413.3351944
ACTGTGC1251.7768434E-613.19889458
GTCTTAA1507.348717E-813.0032651
TATACAG4300.012.9087775
CTAAGAC1056.737717E-512.8589363
GTTCTAG850.002606569812.3560451
CCAGAAC1601.7075581E-712.18923
GTGCTAG900.003998394611.6695971
TACACTG2002.2168024E-811.2515695
TATTAGG2155.3823896E-911.1643492
CTACTTA950.0059836611.05540851
Produced by FastQC (version 0.11.2)