FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_41.351000000390bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_41.351000000390bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739997
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCA400.00650996218.7491999
GACACTC500.001110033817.999237
ATTAGGC805.166863E-616.875423
AGGGGGG550.002100344316.3640425
TATACGG951.5068435E-615.7898655
TAAGACT1052.4737346E-715.7146754
TTGGGGG706.0321233E-415.0003724
GCCTTAG650.006379311413.8474331
TTAGGCT1004.2654872E-513.5003354
TAAGAGT1004.2654872E-513.5003354
ACCGTTC1004.2681975E-513.4994238
GTTATAA1151.0906568E-513.0446831
GTATTAG1507.3572664E-813.0012011
GATCTAC850.002608936312.3540821
TCCTAGA850.002610259212.3532482
TATACTG1702.818706E-812.3532485
TAATACT2800.012.3217334
TAGGTAG1101.0416885E-412.2730315
CCTATTC1354.200887E-612.2225263
TATAGGA1856.9958332E-912.1624642
Produced by FastQC (version 0.11.2)