FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_40.3510000003907a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_40.3510000003907a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336961
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCTC300.001225038124.9990121
AGAGTCC400.00649708518.752048
GTAGGCT400.006502655318.749264
GTGGCTC400.006502655318.7492619
CTAATAC651.6408481E-518.4608083
TATACCG500.001108305217.9992875
GGTTAAT601.8591945E-417.5019051
AATGCAG951.5019123E-615.7888485
CGTGAAC706.016205E-415.00163314
GTGCTAA600.003743877915.0016331
ACGTGAA809.7106225E-515.00163213
AGTGAGC600.003747577314.99940721
CAGTGAG600.003747577314.99940720
GCACTGT600.003747577314.99940729
AGGCAAC650.006369110713.84766110
GTGAGCT650.006375376613.84560622
ATTATAG1352.877423E-713.3328063
TATACAG2151.8189894E-1213.255295
CAGTCTC800.00165082713.1264299
TACAGGC800.001652673613.1244817
Produced by FastQC (version 0.11.2)