FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_39.35100000039037.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_39.35100000039037.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences617878
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAC500.001109574117.9996629
CTGGACT500.001109574117.999664
TGGGGGG703.109163E-517.1425325
TCTGGAC755.6150206E-515.9996993
ACTATGC2000.014.9997178
CCGGATC650.006379019513.84701325
GGTGCGA650.006382440713.84589328
GTATTAG1651.2696546E-913.63610651
GTCTTAT902.649261E-413.3330821
CTGTACT1251.7730017E-613.1997514
GGTCTAG800.001655133613.1247531
ACACGCA954.183219E-412.6313415
TACTATG2401.8189894E-1212.4997647
CCAATAC1702.8170689E-812.3527083
TAGGGGG850.002610273412.3527084
CGCAGAT850.002610273412.35270818
AGGGGGG850.002610273412.3527085
CTATGCA2001.7280399E-911.9997739
TCTATAC1901.03827915E-811.8418823
ACACAGT1151.5764139E-411.7389086
Produced by FastQC (version 0.11.2)