FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_38.35100000039000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_38.35100000039000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681041
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTG550.002098228316.366245
TAGACAG1206.843584E-815.0023865
CCTACTA600.00374785915.0023862
CTATACA952.6280804E-514.2127874
CTACACC1404.4886292E-712.8591884
TCTATGC954.1779154E-412.6335893
AGGTGTG954.1882807E-412.6298787
GTCTATA1101.0394338E-412.2755811
GTCCAGA1101.0394338E-412.2755811
CCTACAC1101.0401471E-412.2746793
TAAGGGT900.00399945911.6685234
TTATTCC1703.7446625E-711.4724144
TGGGGGG950.00598526311.054395
ATGCCAG950.00598526311.054395
TCTAGAC1100.001429483210.9108263
GACAGTT1100.001432992310.9076227
CTAAGAC1253.4124448E-410.8017193
TGTAGAC1253.4124448E-410.8017193
GTATTAC1000.00875580410.5024421
CCCTTGA1000.00875580410.5024421
Produced by FastQC (version 0.11.2)