FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_37.35100000038fb1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_37.35100000038fb1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975667
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCCG500.001106132818.0095085
TAGACAG1303.45608E-1117.3168355
GGCCGAT750.00101881713.99806311
CTAGACA1301.8161336E-713.8534684
GTATTAG2200.013.6435671
TATACCG1004.248072E-513.5071315
CCGAGCC800.001657821913.12318314
GACTCTA1101.043934E-412.2710297
ATACCGT1059.6656434E-411.4346086
GTACTAA1859.078576E-811.35734751
TGGGGGG1355.6149252E-511.116985
CCCTTAG1100.00142604610.9148541
ATAACAC1704.6434707E-610.5938283
AGGGGGG1000.00874119510.5055465
CGAGCCC1000.0087844910.49854815
TAAGCTG1451.15978015E-410.3502925
GCCTTAG1200.002940281310.0052821
TTGGGGG1356.919469E-410.0052824
GGATATA1952.183564E-610.0052811
CTAATAC1809.180241E-610.0052813
Produced by FastQC (version 0.11.2)