FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_36.35100000038f8b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_36.35100000038f8b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504451
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCC200.005145550729.9988129
TGGGGGG550.002100000616.3629885
CGAGACG1306.4392225E-1016.15480623
CTTAAAC901.5786516E-514.9994053
CACGAGA1554.947651E-1014.51699221
CCACGAG1453.0195224E-914.48362120
TCCGAGC1453.0231604E-914.48218413
CCCACGA1504.8676156E-914.00083319
GACGTAG1402.9731382E-813.929399526
CGAGCCC1402.976958E-813.92801915
TTATCCC650.006381178313.8456054
GCCCACG1557.725248E-913.5478518
GAGACGT1454.7111826E-813.44907724
GTATTAG902.6482175E-413.3328051
CTCCGAG1601.2058081E-813.1244812
AGCCCAC1507.3518095E-812.99948417
CCGAGCC1507.3518095E-812.99948414
ACGAGAC1651.8519131E-812.72802922
ATCTCCG1651.8546416E-812.72676810
TGTAGTG1101.0410462E-412.2722422
Produced by FastQC (version 0.11.2)