FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_35.35100000038f48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_35.35100000038f48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908132
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1400.018.2172935
TAGGGGG901.5801677E-515.0024784
CTGTGCG706.0438935E-414.9985469
GCCCACG1054.2776137E-614.284327518
GTCTAGG1501.0367876E-612.003241
CGAGCCC1408.187731E-510.71324715
CCCACGA1451.1684786E-410.34382419
TGGGGGG3101.8189894E-1110.1629695
CCGAGCC1653.899895E-59.9990314
ACCGTGC1200.0029577619.999038
TTATACT6800.09.928114
TAGGACA1552.2841328E-49.6790174
TACACTG4050.09.631225
GTGTGCA3152.5283953E-109.5228859
ACAGATC3152.5283953E-109.5228858
CTCCTAG1901.7516219E-59.4762421
GTAGGAC1603.142122E-49.3765493
CCCTTAT2905.8862497E-99.312861
GTATAAT6800.09.2672071
GTGTAGA4050.09.261761
Produced by FastQC (version 0.11.2)