FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_34.35100000038ef6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_34.35100000038ef6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810360
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG752.7882506E-618.0039625
CAGCGTC805.1584866E-616.8787162
GTAGGAC800.001652931413.127893
GTATAGG1151.088834E-513.0471541
TAGGACC850.002606806612.355664
TACGGTG900.003998757311.6692355
GCGTCAG1059.687505E-411.43108754
GCAGCGT1202.3363669E-411.2531711
AGCGTCA1202.3377055E-411.2524773
TAAACTC1602.2520635E-611.2524775
CTTATGG1202.3377055E-411.2524773
TCTGGAC950.00598420611.0550653
GTGTTAA1100.001428527610.9121661
GGGTAAG1253.4100842E-410.8030441
TTATACT3300.010.4568474
CTTAATC1451.1628452E-410.3471053
GTATATA4800.010.3154071
TAAACAG2053.7518475E-710.2461595
GTACTAG1653.8761893E-510.0028181
CTATACT2003.0272913E-69.7521474
Produced by FastQC (version 0.11.2)