FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_33.35100000038eb2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_33.35100000038eb2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297705
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAC559.552713E-519.0899473
GTATTAG500.001107972817.9990941
TAGACAG600.003746479714.9992465
AGCACTA706.0205586E-414.9992445
TACACTG952.6234711E-514.2098095
TAATACT851.6277334E-414.1169364
GTATAAG1004.2517437E-513.499321
TAAGAAC902.64275E-413.3326623
ATATTAA902.64275E-413.3326621
TGCTAGA800.001652020713.1243392
AATACTG954.1730248E-412.6309425
CCTCAAG850.002605411212.3523191
CTAGACT900.00399667711.6660794
GTATTGA900.00399667711.6660791
CAGCACT900.00399667711.6660794
TATTATA900.00399667711.6660792
GAATTAA900.00399667711.6660791
ATACACA900.00399667711.6660794
TTATACT950.00598118611.0520744
TATTATG1100.001427883710.9085412
Produced by FastQC (version 0.11.2)