FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_32.35100000038e7f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_32.35100000038e7f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716239
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGCC500.001110500317.997999
CCTAGCG854.7139656E-717.6463230
GGACCCT1152.2064341E-916.9629176
TGTTAGG908.5375723E-716.6729532
CCTAATA1603.6379788E-1215.9435112
GACTAGG859.203333E-615.8805798
CACCTAG951.5077258E-615.78881328
CTAGGCA951.5088226E-615.7877110
TGGGGGG901.574272E-515.0056585
TGGACCC1054.2477077E-614.2911015
TAGGGCT952.6230571E-514.2158864
TGGACTG650.006367023613.8513775
TAGGGGG650.006367023613.8513774
AGAGCGG650.006367023613.8513776
ACCCTGC650.00638767513.8446078
ACCTAGC1004.267788E-513.499433529
GACCCTG1004.27059E-513.4984927
CGATCAC1004.27059E-513.49849224
ACACTGG902.6413143E-413.3383636
TTGGGGG902.6413143E-413.3383634
Produced by FastQC (version 0.11.2)