FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_31.35100000038e3c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_31.35100000038e3c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664197
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCG455.4712215E-419.99823824
TGGGGGG1900.017.3736765
TAGGGGG908.5609463E-716.671714
CTAGGTC859.181826E-615.8871594
TAGGGGT1454.7219146E-813.4523454
CCTAGCG1151.0939626E-513.04311330
TAGGACT1702.8174327E-812.3566794
ACGGCGT850.00261483712.3518528
GTATTAG4900.011.9427471
TCTAGAC1901.03864295E-811.8456893
TAGGTAG2553.6379788E-1211.7682665
GTATTAA4600.011.7430351
GTCCTAA1551.5381956E-611.6167661
GACTAGG1551.5460137E-611.611888
TAGACAG2605.456968E-1211.5419525
TAATACT7200.011.46184
TATACAG6150.011.2229075
TTAGGAC1755.4559496E-711.1462293
TTAGGGG1355.6333716E-511.1144733
CTAGGCA1501.3615736E-510.99903110
Produced by FastQC (version 0.11.2)