FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_30.35100000038dea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_30.35100000038dea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1825840
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC1451.8371793E-1015.51555624
TAGGGGG1655.456968E-1215.4592184
CCTAGCG1555.002221E-1014.5165430
TGGGGGG1651.8577339E-812.731125
GTATAGA5650.012.4826741
ACCTAGC1857.024937E-912.16250629
TAGGTAG2351.2732926E-1112.1313265
CGCTCTA900.00401270411.6650810
GTATTAG5300.011.6082351
CTAATAC5400.011.3923323
GACGATC2002.2313543E-811.24877722
AGGGGGG2607.6397555E-1110.9648525
TACGGAC1253.4277313E-410.79853113
GTATTAC4000.010.5040371
TAGACTG2002.6772068E-710.5031745
CCGAGCC1000.00878747710.49885914
TTGGGGG2156.424125E-810.468284
GTATTAA4900.010.4121661
TAATACT9150.010.3309914
GTCTAGT2208.9843525E-810.2312061
Produced by FastQC (version 0.11.2)