FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_29.35100000038d9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_29.35100000038d9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1737369
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG2400.018.1298875
TGCGACG1651.2769306E-913.63650730
CCGAGCC1004.2771695E-513.49858914
GTATTAG4500.013.3373141
CCTAGCG1352.8990144E-713.33347430
TAGGTAG2151.8189894E-1213.2593895
TTGGGGG1753.0613592E-912.8606114
AGGGGGG2102.1827873E-1112.860615
TATACGG4000.012.3783385
ATACGGT3850.012.0811796
GTGCGAC1901.0433723E-811.8422329
TAGACGG1151.5753304E-411.7422965
GTGTATA5500.011.4579641
GACTAGG1501.36148265E-510.99916658
GGAGGCT1100.001434229710.9082638
GTCTAGG1408.1539656E-510.71748351
TGTACCG1551.9496589E-510.6480325
ATAGGGG1551.9502795E-510.6477253
TACGGTG4650.010.6440497
TAGTCAG2402.6575435E-910.6241959
Produced by FastQC (version 0.11.2)