FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_28.35100000038d57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_28.35100000038d57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476043
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG2000.017.2544635
TAGGGGG805.164049E-616.8787924
ACCGTGC851.6345845E-414.1169928
TTGGGGG1651.2714736E-913.6394294
CGGCATA1507.39019E-812.99939725
AGGGGGG1252.567988E-512.0031045
TAGGCTG1006.4368726E-412.0031045
GTCCGTG1355.6524113E-511.1105979
TATACCG3000.011.0028455
TTAGGCT1253.4154748E-410.8024284
TCCGTGG1253.424048E-410.799499510
ACACCGT1951.8891114E-710.7720176
AGGACAG1704.6637215E-610.5909745
TACACCG1304.9043883E-410.3873015
TAGACTG1451.1638729E-410.3475035
TGGACTG1756.592287E-610.2883745
TAAGAGT2651.2423698E-910.1909694
CTAATAC4450.010.1146333
TACCGTG1653.8960978E-59.9995377
CCATGCG1501.6444639E-49.99953659
Produced by FastQC (version 0.11.2)