FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_27.35100000038d13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_27.35100000038d13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850696
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCC200.00514953429.99824513
TGGGGGG2600.015.0015545
CGGCCGG650.00639046813.84534415
TGCGACG1507.387462E-813.00029330
AGACCGA1056.747537E-512.8584756
GTATTAG4750.012.3177311
AGTGCGC1406.323862E-611.7850248
CTTAGGG1151.577874E-411.7403473
GGTGCGA1303.8455622E-511.53872128
GTACTAG2801.8189894E-1211.2517741
CTGATCG950.0060015611.0519859
TAGGTAG2207.7398E-910.9102215
CTAGGAC1100.001432160110.9102213
GTGCGAC1551.955181E-510.64540129
ACTGATC3101.8189894E-1210.6445388
TAGGCTC1000.00877373710.5010885
CGCCGCG1000.00878252710.49966924
TAGACTG1756.607417E-610.2867795
CTAGACT1952.1994456E-610.0010374
CGTGAGC1501.6451786E-49.99941412
Produced by FastQC (version 0.11.2)