FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_26.35100000038cde.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_26.35100000038cde.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258505
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG859.1865095E-615.8856564
TAGACTG1154.0656232E-815.6554295
TAGTCAG1709.094947E-1214.9989489
GGGGTCG600.00375593914.99894714
TAGGTAG1751.2732926E-1114.574465
TACGGAC650.006389463313.84518113
GTCGGGG650.006389463313.84518115
AGTCAGA2400.013.74903510
GTATTAG3950.013.6748161
CCGCCGT1106.915654E-613.63540624
TCCCGCC1004.273955E-513.49905322
GGTAGTC1958.367351E-1113.0760057
AGGGGGG1201.6899417E-512.50265
GCCCACG1456.9032285E-712.41292218
CTAGGAC850.00260842712.3555113
CCCGCCG1101.0437343E-412.27186523
TCCGAGC1406.3201715E-611.78488713
CTCCCGC1151.5796591E-411.73830721
ACGGACA900.004009546711.66584814
CGTACTA1602.2610402E-611.25010529
Produced by FastQC (version 0.11.2)