FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_25.35100000038c9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_25.35100000038c9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601462
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTTAG400.006485030518.7609711
CCTAATA1700.016.773122
TAGGTAG653.4222755E-416.160615
ACCTAAT1707.2759576E-1215.0087781
GTACTAA809.691402E-515.0087771
AGTCAGA1002.572926E-614.99754710
TATTAGG1054.239493E-614.2928852
GTCTTCG851.6336686E-414.115349
GATCTAG650.006356697513.8542571
ACTGGTC650.006388296413.843898
CTAGGCA902.6523226E-413.33115310
GTCTTAC800.00164753513.132681
TGGGGGG800.001649600713.1304955
CACCTAG800.001654774813.12503628
TACTATG1753.0649971E-912.85504157
CTATGCA1651.8604624E-812.7251919
GTATTAG1302.734192E-612.6997351
ATTAACC954.167519E-412.6368683
CGTGAGC954.188035E-412.62951512
TAATACT3150.012.3861364
Produced by FastQC (version 0.11.2)