FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_24.35100000038c58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_24.35100000038c58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655398
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGA13730.20949102682644746No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAG6990.10665275145789277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGCA559.574143E-519.0901819
GGATAGC400.006494689718.7564411
TAGGGGG500.001108679218.002064
ATAGGGG1101.2241799E-917.7293013
TAGAGGA3150.017.61837630
ACTTCGC550.002100882416.3630128
GTTCGCT550.002100882416.3630129
GTAGAGG3300.016.36301229
TGACGCG653.4347954E-416.1532297
GACGTAG3350.015.67104426
TAGAACT1451.8189894E-1015.5190184
CTCTTAT1604.9112714E-1115.0051531
CATACTC809.732477E-515.0017183
TAGGACA1002.566354E-615.0017174
TACCCGG706.0336996E-414.99942822
GCGCATG809.745147E-514.99942811
ACTCCCG600.003752580614.99942820
CGAGACG3650.014.79395723
CCGAGCC3550.014.78816914
ATAATAC2150.014.6528413
Produced by FastQC (version 0.11.2)