FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_23.35100000038c21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_23.35100000038c21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1646944
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCG1253.7107384E-1016.79938730
TAGGTAG2700.016.1144375
TGGGGGG1750.015.4317575
ACCTAGC1503.0559022E-1014.99945329
CGATCAC1401.8553692E-914.99899924
GTATTAG4450.014.8354241
AGGGGGG2250.014.6696965
GCGCCGA1156.949431E-714.34773926
CCCGCCG851.6350501E-414.11670423
GGTAGTC3100.014.0308947
CCCCGTG650.00638913813.84564930
GGCGCCG1106.9141715E-613.63628125
TATTAGG4100.013.5397922
TAGGGGG1454.7277354E-813.4510534
CTATGCA4300.012.9061159
TCCCGCC954.1914883E-412.63073522
TAGTCAG3450.012.6078539
CCTAATA5500.012.0028432
CACCTAG1901.043918E-811.84167428
GCCGCAG1151.5801759E-411.73834717
Produced by FastQC (version 0.11.2)