FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_22.35100000038bec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_22.35100000038bec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777259
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTGT1450.017.5855278
TAGTCAG1202.0736479E-1017.4993259
CCTAGCG653.4375198E-416.15218430
GACTAGG755.6189587E-515.9993828
TTAGGTA1750.015.43294054
CTAGGCA706.035509E-414.99942110
CGATCAC809.754247E-514.99845524
GGTAGTC1351.850458E-814.4438867
TAGGGGG851.628449E-414.1216464
TAGGACA851.628449E-414.1216464
ACACCGC650.00636891913.8509666
TACGGTG1955.456968E-1213.845627
GACTGAG650.00638522913.8456197
GACTACG650.00638522913.8456197
TTAAGAC1201.117347E-613.7538943
ACCGTGT1004.2685737E-513.4994788
TAGAGCG902.6424305E-413.3379685
TAAGACT902.6438705E-413.3371094
TAGGTAG1701.9772415E-913.2398945
TAGACTG800.001651241413.1295615
Produced by FastQC (version 0.11.2)