FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_21.35100000038ba9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_21.35100000038ba9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130687
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCT1440.11018693519630875No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGC200.00511956230.0134052
GCCCGGT200.005129163630.00191315
GCAAGCA200.00513877929.99043324
GAGTTAC300.001217563325.0111711
CTACGCT300.001220286725.00159630
GCTACGC300.001220286725.00159629
CCCGGTT300.001220286725.00159616
GTTTTAT402.4438946E-422.5100541
AAGTGTG402.456607E-422.4928257
GTACAAG350.002984321721.4381471
GTCTAAG350.002984321721.4381471
ACCGTCG350.002990964621.4299428
AAGGTGC400.006463654318.7583796
TTGAACT400.006463654318.7583794
CCGAGCC400.00647797218.75119614
GACGCTA400.00647797218.75119626
GGCCATG400.00649231618.744027
TAAGGTG500.001099771818.0080435
TATACAC500.001099771818.0080433
GTTGAAC705.9659564E-415.0067023
Produced by FastQC (version 0.11.2)