FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_20.35100000038b65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_20.35100000038b65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353229
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCAAC350.003003149321.42725610
CTAGGCA500.001108610717.99889810
CTAATAC805.148755E-616.8763543
GTATAAT1154.0363375E-815.6556451
GTTCTGG706.012066E-415.0033261
TAGACCG1002.5591453E-615.0012045
TTATAGG706.018532E-415.0012042
CTAACAC600.00374506715.0012043
AGCTCCA706.0250034E-414.9990810
TGTAGAC1156.8981353E-714.3489763
GTATTAG952.6194706E-514.2136791
CTACACC952.6229718E-514.2116664
CCTAATA750.001014879214.0011232
TATAATA1504.8457878E-914.0011232
GAGAGCT750.001015966313.9991417
CTGCCCT750.001015966313.99914111
TCACCGA650.006371120513.84726529
GGAGAGT650.006371120513.8472656
ACTACAC650.006371120513.8472653
TCTGGGC650.006371120513.8472653
Produced by FastQC (version 0.11.2)