FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_19.35100000038b22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_19.35100000038b22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747769
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG702.3628672E-923.5759934
GGCGCCG952.6368514E-514.2104325
TAGGTAG2950.013.7314725
GTATTAG5500.013.6405661
TATTAGG4900.013.4719962
CTGGGCG902.6552603E-413.33219
CCTAGCG1601.2129021E-813.12491330
CCGGCTG954.1895945E-412.63149414
ACTGAGC1804.6838977E-912.4988438
CTATGCA5050.012.1770929
CTGGCGC1006.4569473E-411.9988923
GGTAGTC3150.011.903327
CCTAATA6250.011.7622782
GTAGCGT1151.5764566E-411.7414036
CACTCCG1151.5793987E-411.73905114
GTCGCTT2301.05501385E-1011.73804411
CTATACT4550.011.5406964
ATCACAC4450.011.46092529
AGGGGGG2103.7307473E-911.4307845
TAGTCAG3150.011.4275159
Produced by FastQC (version 0.11.2)