FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_18.35100000038aed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_18.35100000038aed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2078795
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGGG802.5002737E-718.74914215
CGGTCGG951.5111582E-615.78875213
CCTAGCG2000.015.00039830
TCGGGGG1002.5771533E-614.99931416
TAGTCAG3200.014.0611819
TAGGTAG4200.013.9316215
CGATCAC2200.013.63508424
GGTAGTC3650.013.1494517
TGGGGGG2550.012.944015
GTATTAG6350.012.7593131
GGTCGGG1551.13475835E-712.5800714
CGGGGGC1201.6956057E-512.49942917
GACGATC2650.012.45166222
TAGGGGG1456.886312E-712.4165124
ACCTAGC2550.012.35326929
TATTAGG5750.012.0023392
CACCTAG2950.011.69522528
TAATACT10600.011.6063154
TCGCCCG1202.3478856E-411.24894524
CATATAC8150.011.2291813
Produced by FastQC (version 0.11.2)