FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_17.35100000038ab6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_17.35100000038ab6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568154
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCA4700.016.5955169
TGGGGGG2000.016.5039835
CTGTCCG653.4379758E-416.1536249
GTATTAG4950.016.0649931
TAGTCAG2450.015.9181489
ATCACAC4200.015.71356829
TAGGTAG2950.015.2579195
ACTATGC5550.014.5943938
CTCGCCG952.6382731E-514.20942318
CCTAATA6300.014.0510092
TCACACA4700.014.04191230
GGTAGTC2900.013.9648797
CTAATAC7000.013.9314893
TTAGGTA4350.013.7964334
AGGGGGG1853.45608E-1113.7871095
TATTAGG4750.013.2663582
GCGACGC800.001657844813.12398122
CGCCGAG800.001657844813.12398120
CCTAGCG1151.0940659E-513.04288230
TAATACT9300.012.9063394
Produced by FastQC (version 0.11.2)