FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_16.35100000038a73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_16.35100000038a73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1739743
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATCG752.8004943E-617.9982249
TGGGGGG2150.016.0513885
CCTAGCG1553.092282E-1115.48412330
TAGGGGG1251.129647E-714.4043754
CGATCAC1802.1827873E-1114.16526924
TGCCGGA1004.27694E-513.49866910
CTATGCA5000.013.1986979
CCTACAC1601.2076271E-813.1289873
CTAGGCA1958.367351E-1113.07563210
ATCACAC4200.012.50020329
GTAGGGG1456.8770896E-712.4175643
GTATTAG4900.012.248971
TAATACT9500.012.1615894
GTCTAGA2102.8740033E-1012.1468951
ACTATGC6450.012.091838
ACCTAAT5600.011.7896341
GTCCTAA2206.2573235E-1011.5947651
CTAAGAC1951.5257683E-811.5419673
CTAATAC7550.011.526683
GACGTGC1059.6904876E-411.4320446
Produced by FastQC (version 0.11.2)