FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_15.35100000038a30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_15.35100000038a30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762238
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG1301.8266837E-713.8486554
TAGGTAG3350.012.9874225
CCTACAC1601.7109232E-712.1893563
CGGGGGG1006.442186E-412.0021695
GGACTGC1151.5766606E-411.7412516
GCCGGGG1059.716743E-411.4283678
TTAGGTA4100.011.3435134
CCGGGGC2053.205969E-810.9754129
AGGGGGG1408.163872E-510.7162225
CGGGGCC2104.5693014E-810.71409310
CTATACT5350.010.6561314
CCGTCTG1000.00878136810.4998119
CGCCCCC1000.00878321510.49951419
GGTCGGG1150.00207649410.434298514
GCCGATA1150.00207649410.434298512
TACACAG2752.1464075E-1010.3655095
CCGTTCC1451.16742915E-410.3446429
TAGTCAG2505.1968527E-910.1998179
GTATTAG4600.010.1102351
CTAGCCT1653.8875503E-510.0018064
Produced by FastQC (version 0.11.2)