FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_14.351000000389fb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_14.351000000389fb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607427
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG550.002098844416.3674745
CTAGCCT952.6306052E-514.2138584
GTCCGGC1056.7598434E-512.85575611
CCTAGAC1354.1996955E-612.2250873
TCCGGCC1354.215788E-612.22090412
AGGGGGG1252.5689593E-512.0028135
CCGGCCC1151.5802766E-411.7382313
TCTATAC4650.011.292973
ACTGCCC1202.3475623E-411.2487878
CGGCCCG1355.6548874E-511.11025914
GGCCCGC1253.4253695E-410.79917115
ACTGCGT1253.426355E-410.7988368
TACTATG5150.010.4843067
GTATTAG4300.010.4685471
ACTATGC3450.010.4336578
CTAATAC3750.010.4024373
TAGACCG1451.1643059E-410.3472525
TAATACT6750.010.2246194
GTCTATG2505.16593E-910.2033431
CCAATAC4350.010.0023443
Produced by FastQC (version 0.11.2)