FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l03n01_ntr_13.351000000389b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l03n01_ntr_13.351000000389b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919008
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCG901.5832717E-514.9993759
GCTCCCC650.006389867513.84557611
TAGGCTG1201.691297E-512.5024115
GTATTAG5250.012.0023151
GGATAGG1459.319352E-611.3815051
GTCTTAG2002.2217137E-811.252171
TAGGACT1602.2587938E-611.2518774
TATACTG7650.010.982515
CCGCGTC1408.183193E-510.7138418
CGCGTCG1408.183193E-510.7138419
CAGGACC1851.1188458E-610.5422984
GGTAGTC2853.8198777E-1110.5258777
GCCTAGA2002.6815542E-710.5020251
GTGTAGG1602.770622E-510.3144891
TAGGTAG3253.6379788E-1210.1558045
AGACTGA3401.8189894E-1210.1490156
GACAGGG1356.9623237E-49.9995837
CCATGCG1653.8975566E-59.9995839
ACTGATC2403.0044248E-89.9995828
TAATACT9900.09.8501274
Produced by FastQC (version 0.11.2)